Level Up Your 3D Cell Culture: From Research to High Throughput

In the last several years, multiple research publications have shown the increased value of using 3D cell models compared to traditional 2D systems. Although considered by many as a more representative model of in vivo biology, the setup of 3D models can be more challenging, especially when the throughput is increased. This course will provide an overview of scaffold and scaffold-free techniques and key optimizations for increased throughput through imaging technologies and automation. Attendees will learn the tips and tricks for improving cell seeding, handling, processing and use of high-content imaging and analysis for 3D applications to address the need of higher throughput demands.

Who Should Attend?

• Anyone new to the topic of 3D cell culture
• Those performing 3D at a research scale looking to increase throughput

Course Benefits

This course will provide educational information and practical techniques to move the valuable, more predictive 3D cell culture models from more manual research-based work to a more automated, mid-to high-throughput environment.

Course Topics

• 3D Culture Models
• Scaffold-Free vs. Hydrogel Matrix Models
• High-throughput Imaging
• High-throughput Liquid Handling
• Bioprinting
• Organoids
• Screening

Instructors

Hilary Sherman
Corning Life Sciences

Hilary Sherman is a Senior Scientist in the Corning Life Sciences Applications Lab located in Kennebunk, ME. Sherman has been with Corning Incorporated since 2005 and has worked with a wide variety of cell types including mammalian, insect, primary, stem cells and organoids in a vast array of applications. Her key roles at Corning involve creating technical documents such as protocols and whitepapers as well as providing technical support and training for both the Corning sales force and customers. Hilary received her B.S. degree in Biology from the University of New Hampshire. In the last several years, Sharman has focused on 3D cell culture applications including human organoid culture.

Oksana Sirenko, Ph.D.
Molecular Devices

 

Dr. Sirenko is an established cell biologist and imaging specialist who is an expert in developing assays with complex cell-based models for research and drug discovery. She is a senior scientist at Molecular Devices where she works on development of high-content imaging methods to the analysis of novel cell systems. She currently leads a group of scientists developing methods and new tools for automation of 3D cell models – including organoids and organ-on chips – for modeling cancer, neurotoxicity, and toxicology. Sirenko holds a PhD in Biochemistry/Biophysics, has over 15 years of industry experience, and has authored more than 35 scientific papers