High-content screening is a powerful technology platform for implementing functional cell-based assays that allow truly multi-parametric analysis in the physiological context of intact cells, including 3D models. This half-day course provides an introductory level overview of the state-of-the-art components of HCS including the wide variety of imagers, good practice in cell-based assay development and some showcases of high-content screens in industry and academia. An outlook to the second part of this Short Course on image and data analysis will be provided.
Who Should Attend?
- Individuals who plan to develop or apply high-content assays and screening technologies
- Individuals who look to implement HCA/HCS infrastructure and capability at their organization or labs
- Individuals with first experience in HCA/HCS but want to get a systematic overview, learn more about good practice in assay development and screening.
Course Benefits
- Get an introduction to all aspects of HCS with a focus on imagers, assay development and screening.
- Get an overview on major categories and thereof examples of HCS instrumentation
- Attain guidance to assay selection and systematic optimization
- Learn the benefits and challenges of more complex assays for screening such as 3D models will be discussed.
- See a demonstration of good practice in assay validation and screening
- Learn considerations on strategies, screening, quality assurance and hit verification
- Participate in a presentation of case studies of assay development and screens
Course Topics
- Introduction to High-Content Analysis and Screening (HCA / HCS)
- HCS Instrumentation Overview
- Reagents, Cells, Assays and Assay Development for HCS
- Screening, Hit Finding and Hit Verification
- Case Studies of High-Content RNAi and Small Molecule Screens
Instructors
Eberhard Krausz, Ph.D.
ChemBioCon (Germany)
